Abstract
Sample-induced image-degradation remains an intricate wave-optical problem in light-sheet microscopy. Here we present biobeam, an open-source software package that enables simulation of operational light-sheet microscopes by combining data from 105-106 multiplexed and GPU-accelerated point-spread-function calculations. The wave-optical nature of these simulations leads to the faithful reproduction of spatially varying aberrations, diffraction artifacts, geometric image distortions, adaptive optics, and emergent wave-optical phenomena, and renders image-formation in light-sheet microscopy computationally tractable.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Computational Biology
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Computer Simulation
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Image Enhancement / methods
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Image Processing, Computer-Assisted / methods
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Image Processing, Computer-Assisted / statistics & numerical data
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Imaging, Three-Dimensional / methods
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Imaging, Three-Dimensional / statistics & numerical data
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Light
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Microscopy, Fluorescence / methods*
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Microscopy, Fluorescence / statistics & numerical data*
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Optical Phenomena
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Scattering, Radiation
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Software*
Grants and funding
Funding was provided by the Max Planck Society and the Bundesministerium für Bildung und Forschung (BMBF, grant number 031L0044). The authors are responsible for the content of this publication. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.