In Vivo Experimental Study of Biological Compatibility of Tissue Engineered Tracheal Construct in Laboratory Primates

Bull Exp Biol Med. 2018 Apr;164(6):770-774. doi: 10.1007/s10517-018-4077-y. Epub 2018 Apr 16.

Abstract

Biological compatibility of a tissue engineered construct of the trachea (synthetic scaffold) and allogenic mesenchymal stem cells was studied on laboratory Papio hamadryas primates. Subcutaneous implantation and orthotopic transplantations of tissue engineered constructs were carried out. Histological studies of the construct showed chaotically located filaments and mononuclear cells fixed to them. Development of a fine connective tissue capsule was found at the site of subcutaneous implantation of the tissue engineered construct. The intact structure of the scaffold populated by various cell types in orthotopic specimens was confirmed by expression of specific proteins. The results indicated biological compatibility of the tissue engineered construct with the mesenchymal stem cells; no tissue rejection reactions were recorded; simulation of respiratory disease therapy on Papio hamadryas proved to be an adequate model.

Keywords: mesenchymal stem cells; primates; subcutaneous test; tissue engineered tracheal construct; transplantation.

MeSH terms

  • Animals
  • Biomarkers / metabolism
  • Cell Adhesion / drug effects
  • Foreign Bodies / surgery*
  • Gene Expression
  • Keratins / genetics
  • Keratins / metabolism
  • Lectins, C-Type / genetics
  • Lectins, C-Type / metabolism
  • Leukocytes, Mononuclear / cytology
  • Leukocytes, Mononuclear / drug effects
  • Leukocytes, Mononuclear / metabolism
  • Male
  • Mannose Receptor
  • Mannose-Binding Lectins / genetics
  • Mannose-Binding Lectins / metabolism
  • Mesenchymal Stem Cell Transplantation*
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / metabolism
  • Papio hamadryas
  • Polyethylene Terephthalates / pharmacology*
  • Receptors, Cell Surface / genetics
  • Receptors, Cell Surface / metabolism
  • Subcutaneous Tissue / surgery
  • Tissue Engineering / methods*
  • Tissue Scaffolds*
  • Trachea / transplantation*
  • Transplantation, Autologous
  • Vimentin / genetics
  • Vimentin / metabolism

Substances

  • Biomarkers
  • Lectins, C-Type
  • Mannose Receptor
  • Mannose-Binding Lectins
  • Polyethylene Terephthalates
  • Receptors, Cell Surface
  • Vimentin
  • Keratins