Abstract
We studied expression profile of microRNA and their target genes in human umbilical vein endothelial cells (HUVEC) during proinflammatory activation with TNFα. TNFα-induced activation of HUVEC was accompanied by a decrease in the expression of CDKN1B, HIST1H3D, and OIP5 genes that are the common target genes for mature microRNA encoded by MIR221, MIR222, and MIR181B1 genes, whose expression increases in activated cells. Proteins encoded by HIST1H3D and OIP5 genes are associated with chromatin compaction and cell cycle. Our results suggest that fetal endothelial microRNA can appear in the maternal blood during various pathological states, e.g., under conditions of preeclampsia.
Keywords:
HUVEC; TNFα; barrier; microRNA; microarray analysis.
MeSH terms
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Binding Sites
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Cell Cycle Proteins
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Chromosomal Proteins, Non-Histone / genetics
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Chromosomal Proteins, Non-Histone / metabolism
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Cyclin-Dependent Kinase Inhibitor p27 / genetics
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Cyclin-Dependent Kinase Inhibitor p27 / metabolism
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Endothelium, Vascular / drug effects
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Endothelium, Vascular / metabolism
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Endothelium, Vascular / pathology
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Gene Expression Regulation
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Histones / genetics
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Histones / metabolism
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Human Umbilical Vein Endothelial Cells / drug effects*
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Human Umbilical Vein Endothelial Cells / metabolism
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Human Umbilical Vein Endothelial Cells / pathology
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Humans
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Inflammation
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MicroRNAs / agonists
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MicroRNAs / genetics*
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MicroRNAs / metabolism
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Models, Biological
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Primary Cell Culture
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Signal Transduction
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Tumor Necrosis Factor-alpha / pharmacology*
Substances
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Cell Cycle Proteins
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Chromosomal Proteins, Non-Histone
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Histones
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MIRN221 microRNA, human
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MIRN222 microRNA, human
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MIrn181 microRNA, human
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MicroRNAs
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OIP5 protein, human
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Tumor Necrosis Factor-alpha
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Cyclin-Dependent Kinase Inhibitor p27