Abstract
The aziridinium ion of ethylcholine (AF64A) is a neurotoxin that has demonstrated selectivity for cholinergic neurons. Unilateral stereotaxic injection of AF64A into the caudate-putamen of rats, resulted in a decrease in dopamine D-2 receptors as evidenced by a decrease in [3H]-sulpiride binding. Dopamine D-1 receptors, labeled with [3H]-SCH 23390, were unchanged. The efficacy of the lesion was demonstrated by the reduction of Na+-dependent high affinity choline uptake sites labeled with [3H]-hemicholinium-3. These data indicate that a population of D-2 receptors are postsynaptic on cholinergic interneurons within the striatum of rat brain.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Aziridines / pharmacology
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Caudate Nucleus / drug effects
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Caudate Nucleus / metabolism*
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Choline / analogs & derivatives
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Choline / pharmacology
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Choline / physiology*
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Hemicholinium 3 / metabolism
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Interneurons / metabolism*
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Male
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Putamen / drug effects
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Putamen / metabolism*
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Rats
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Rats, Inbred Strains
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Receptors, Dopamine / drug effects
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Receptors, Dopamine / metabolism*
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Receptors, Dopamine D1
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Receptors, Dopamine D2
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Sulpiride / metabolism
Substances
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Aziridines
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Receptors, Dopamine
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Receptors, Dopamine D1
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Receptors, Dopamine D2
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Hemicholinium 3
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Sulpiride
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ethylcholine aziridinium
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Choline