Direct E-DNA sensor of Mycobacterium tuberculosis mutant strain based on new nanocomposite transducer (Fc-ac-OMPA/MWCNTs)

Salma Bizid; Saber Blili; Rym Mlika; Ayoub Haj Said; Hafsa Korri-Youssoufi

doi:10.1016/j.talanta.2018.03.025

Direct E-DNA sensor of Mycobacterium tuberculosis mutant strain based on new nanocomposite transducer (Fc-ac-OMPA/MWCNTs)

Talanta. 2018 Jul 1:184:475-483. doi: 10.1016/j.talanta.2018.03.025. Epub 2018 Mar 14.

Authors

Salma Bizid¹, Saber Blili², Rym Mlika³, Ayoub Haj Said², Hafsa Korri-Youssoufi⁴

Affiliations

¹ Laboratory of Interfaces and Advanced Materials, Faculty of Science of Monastir, University of Monastir, 5019 Monastir, Tunisia; UMR-CNRS, Institute of Molecular Chemistry and Materials of Orsay, Bioorganic and Bioinorganic Chemistry Team, University Paris-Sud, Building 420, 91405 Orsay, France.
² Laboratory of Interfaces and Advanced Materials, Faculty of Science of Monastir, University of Monastir, 5019 Monastir, Tunisia.
³ Laboratory of Interfaces and Advanced Materials, Faculty of Science of Monastir, University of Monastir, 5019 Monastir, Tunisia. Electronic address: [email protected].
⁴ UMR-CNRS, Institute of Molecular Chemistry and Materials of Orsay, Bioorganic and Bioinorganic Chemistry Team, University Paris-Sud, Building 420, 91405 Orsay, France.

PMID: 29674071
DOI: 10.1016/j.talanta.2018.03.025

Abstract

Direct DNA sensor based on new nanocomposite materials (Fc-ac-OMPA/MWCNTs) has been investigated. This nanocomposite was formed by combining the redox oligomer "oligo-methoxy-phenyl-acetonitrile" (Fc-ac-OMPA) and the MWCNTs via a simple π-π stacking interaction in the aim to ameliorate the biosensor performance. The redox indicator and the functional groups of the redox oligomer have been used for monitoring the electrochemical behavior and the flexibility for direct covalent attachment of Hepatitis C DNA probe. This nanocomposite shows high performance of DNA hybridization with a detection limit of 0.08 fmol L^-1. Moreover, the biosensor was applied for the detection of pathogenic bacterium such as DNA from Mycobacterium tuberculosis strand. Developed biosensor has been able to detect a single nucleotide polymorphism (SNP)T (TCG/ TTG) which confers resistance of M. tuberculosis to rifampicin drug.

Keywords: DNA biosensor; Electrochemistry; MWCNTs; Poly(p-phenylene); Redox oligomer.

MeSH terms

Biosensing Techniques*
DNA, Bacterial / chemistry*
Drug Resistance, Bacterial / drug effects
Microbial Sensitivity Tests
Mutation
Mycobacterium tuberculosis / drug effects
Mycobacterium tuberculosis / genetics*
Mycobacterium tuberculosis / isolation & purification
Nanocomposites / chemistry*
Polymorphism, Single Nucleotide / genetics
Rifampin / pharmacology

Substances

DNA, Bacterial
Rifampin