Genome-wide profiles of metastasis-associated mRNAs and microRNAs in salivary adenoid cystic carcinoma

Shubin Wang; Lei Zhang; Pengwei Shi; Yuanyuan Zhang; Hong Zhou; Xuanping Cao

doi:10.1016/j.bbrc.2018.04.122

Genome-wide profiles of metastasis-associated mRNAs and microRNAs in salivary adenoid cystic carcinoma

Biochem Biophys Res Commun. 2018 Jun 7;500(3):632-638. doi: 10.1016/j.bbrc.2018.04.122. Epub 2018 Apr 26.

Authors

Shubin Wang¹, Lei Zhang², Pengwei Shi¹, Yuanyuan Zhang¹, Hong Zhou¹, Xuanping Cao³

Affiliations

¹ Department of Stomatology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
² Institute of Biomedical and Pharmaceutical Sciences, and Provincial Cooperative Innovation Center, College of Bioengineering, Hubei University of Technology, Wuhan, China.
³ Department of Stomatology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China; Academy of Medical Sciences, Zhengzhou University, Zhengzhou, China. Electronic address: [email protected].

PMID: 29678584
DOI: 10.1016/j.bbrc.2018.04.122

Abstract

Salivary adenoid cystic carcinoma (SACC) is often accompanied with poor prognosis due to local recurrence, distant metastasis, and perineural invasion. The mechanism involved in SACC metastasis is not yet fully understood. In this study, we profiled the expression of messenger RNA (mRNA) and microRNA (miRNA) in a SACC cell line, ACC-2, and a highly metastatic SACC cell line, ACC-M, using high-throughput sequencing. We discovered that: (1) differentially expressed (DE) mRNAs and DE miRNAs are potentially involved in SACC metastasis; (2) multiple regulatory interactions between DE miRNAs and DE mRNAs exist; and (3) miR-338-5p/3p target LAMC2 to impair motility and invasion of ACC-M and MDA-MB-231 cells. In conclusion, our study integrated the regulatory effects of miRNAs and mRNAs on SACC metastasis and provided a potential application for miRNAs in future therapeutic intervention.

Keywords: LAMC2; Metastasis; RNA-seq; Salivary adenoid cystic carcinoma; miR-338.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Carcinoma, Adenoid Cystic / genetics*
Carcinoma, Adenoid Cystic / pathology*
Cell Line, Tumor
Gene Expression Profiling*
Gene Expression Regulation, Neoplastic
Gene Regulatory Networks
Genome, Human*
Humans
MicroRNAs / genetics*
MicroRNAs / metabolism
Neoplasm Metastasis
RNA, Messenger / genetics
RNA, Messenger / metabolism
Salivary Gland Neoplasms / genetics*
Salivary Gland Neoplasms / pathology*

Substances

MicroRNAs
RNA, Messenger