In Vitro Evolution and Selection of Proteins: Ribosome Display for Larger Libraries

Ulrich Hoffmüller; Jens Schneider-Mergener

doi:10.1002/(SICI)1521-3773(19981217)37:23<3241::AID-ANIE3241>3.0.CO;2-2

In Vitro Evolution and Selection of Proteins: Ribosome Display for Larger Libraries

Angew Chem Int Ed Engl. 1998 Dec 17;37(23):3241-3243. doi: 10.1002/(SICI)1521-3773(19981217)37:23<3241::AID-ANIE3241>3.0.CO;2-2.

Authors

Ulrich Hoffmüller¹, Jens Schneider-Mergener¹

Affiliation

¹ Institut für Medizinische Immunologie, Universitätsklinikum Charité, Humboldt-Universität, Schumannstrasse 20/21, D-10098 Berlin (Germany), Fax: (+49) 30-2802-6460.

Abstract

The cell-free production of large protein libraries can now be achieved with the ribosome display technique. The use of intact ribosomes (R) in the translation of a mRNA library enables the rapid selection of the generated proteins (P) according to their affinity to a given immobilized binding partner (B).

Keywords: Combinatorial chemistry; In vitro selection; Nucleic acids; Proteins; Ribosome display.