Not "from above", but "from the side": Configuration-retaining β-glycosidases protonate their substrate either anti or syn to the endocyclic C1-O bond as the first step in the enzymic cleavage of the glycosidic bond (see schematic drawing). Insights into the mechanism of action of glycosidases have been gained by a combination of the synthesis of inhibitors, the study of the kinetics of their inhibition, and the analysis of the crystal structures of glycosidases and glycosidase-ligand complexes.
Keywords: Azoles; Enzyme inhibitors; Glycosidases; Lactams; Protonations.
© 1999 WILEY-VCH Verlag GmbH, Weinheim, Fed. Rep. of Germany.