Objective: To investigate the expression of phosphoglycerate kinase 1 (PGK1) and its prognostic value in endometrial carcinoma (EC).
Methods: The expression of PGK1 was detected immunohistochemically in 30 normal endometrium and 130 EC specimens. The relationship between PGK1 protein expression and the clinicopathological features of the patients was evaluated.
Results: Immunohistochemical analysis revealed low PGK1 expression in 55.4% (72/130) and high PGK1 expression in 44.6% (58/130) of the EC specimens, as compared with the rates of 90% (27/30) and 10% (3/30) in normal endometrium, respectively (P<0.001). PGK1 expression was significantly correlated with FIGO stage (P<0.001), histological grade (P=0.002) and lymph node metastasis (P<0.001). Kaplan-Meier survival analysis indicated that patients with a high PGK1 expression had a shorter overall survival rate than those with a low PGK1 expression (P<0.001). Multivariate analysis showed that a high PGK1 expression was not the independent predictor of the prognosis of EC (P=0.077).
Conclusion: A high expression of PGK1 is associated with aggressive and metastatic behaviors of EC, and detection of PGK1 provides assistance in evaluating the prognosis of patients with EC.
目的: 探讨子宫内膜癌组织中PGK1的表达情况及其对患者预后的影响。
方法: 运用免疫组织化学SP法检测30例正常子宫内膜组织和130例子宫内膜癌组织中PGK1的表达，分析PGK1蛋白的表达与子宫内膜癌临床病理参数的关系及对子宫内膜癌预后的影响。
结果: （1）30例正常子宫内膜组织标本中PGK1低表达27例（90%），高表达3例（10%），130例子宫内膜癌组织标本中，PGK1低表达72例（55.4%），高表达58例（44.6%），相对于正常子宫内膜组织，PGK1在子宫内膜癌组织中表达显著，有统计学差异（P < 0.001）；（2）PGK1的表达在子宫内膜癌不同FIGO分级（P < 0.001），组织学分级（P=0.002），淋巴结转移状态（P < 0.001）中差异显著；（3）生存曲线分析显示，PGK1高表达患者的生存时间少于低表达患者（P=0.002）；（4）多因素分析提示，PGK1高表达不是子宫内膜癌患者预后的独立影响因素（P=0.077）。
结论: PGK1高表达与子宫内膜癌的生长浸润行为相关，且与患者预后密切相关。通过检测PGK1的表达能够更加有效地评估子宫内膜癌患者的预后。