Objective: To determine the influence of isoforms of transferrin (Tf) on the detection of serum carbohydrate-deficient transferrin (CDT) by capillary electrophoresis (CE).
Methods: A total of 51,17,and 65 serum samples were collected from the healthy participants,the patients with non-alcoholic liver diseases,and the patients with alcoholic liver diseases,respectively. Serum CDT was measured by CE. The genotype ofTf of the samples without a good separation was further analyzed using high resolution melting (HRM) methods. Those with suspected mutation were confirmed by sequencing.
Results: Six samples showed incomplete separation (2 from the healthy participants,3 from the patients with non-alcoholic liver diseases,1 from the patient with alcoholic liver diseases). Of the 133 serum samples,2.3% (3/133) were detected withTf-Dchi heterozygote. But noTf-D1 andTf-B2 heterozygote were identified.
Conclusion: Tf-Dchi variant influences the measurement of carbohydrate deficientTf by CE,leading to unreliable results.
Keywords: Alcoholism; Capillary electrophoresis; Carbohydrate-deficient transferrin; Gene polymorphism; Transferrin.
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