Experimental and Computational Protocols for Studies of Cross-Seeding Amyloid Assemblies

Baiping Ren; Rundong Hu; Mingzhen Zhang; Yonglan Liu; Lijian Xu; Binbo Jiang; Jie Ma; Buyong Ma; Ruth Nussinov; Jie Zheng

doi:10.1007/978-1-4939-7811-3_27

Experimental and Computational Protocols for Studies of Cross-Seeding Amyloid Assemblies

Methods Mol Biol. 2018:1777:429-447. doi: 10.1007/978-1-4939-7811-3_27.

Authors

Baiping Ren¹, Rundong Hu¹, Mingzhen Zhang¹, Yonglan Liu¹, Lijian Xu^{1

2}, Binbo Jiang^{1

3}, Jie Ma^{1

4}, Buyong Ma⁵, Ruth Nussinov^{6

7}, Jie Zheng⁸

Affiliations

¹ Department of Chemical & Biomolecular Engineering, The University of Akron, Akron, OH, USA.
² College of Life Sciences and Chemistry Hunan University of Technology, Zhuzhou, China.
³ College of Chemical and Biological Engineering, Zhejiang University, Zhejiang, Hangzhou, China.
⁴ State Key Laboratory of Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Tongji University, Shanghai, China.
⁵ Basic Science Program, Leidos Biomedical Research, Inc., Cancer and Inflammation Program, National Cancer Institute, Frederick, MD, USA.
⁶ Basic Science Program, Leidos Biomedical Research, Inc., Cancer and Inflammation Program, National Cancer Institute, Frederick, MD, USA. [email protected].
⁷ Sackler Institute of Molecular Medicine, Department of Human Genetics and Molecular Medicine Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel. [email protected].
⁸ Department of Chemical & Biomolecular Engineering, The University of Akron, Akron, OH, USA. [email protected].

Abstract

Alzheimer's disease (AD) and type 2 diabetes (T2D) are two common protein aggregation diseases. Compelling evidence has shown a link between AD and T2D, which may derive from interspecies cross-sequence interactions between amyloid-β peptide (Aβ), associated with AD, and human islet amyloid polypeptide (hIAPP), associated with T2D. Herein, we present experimental and computational protocols and tools to study the aggregate structures and kinetics, conformational conversion, and molecular interactions of Aβ-hIAPP mixtures. These protocols could be generally applied to other cross-seeding behaviors of amyloid peptides.

Keywords: Alzheimer disease; Amyloid peptides; Aβ; Cross-seeding; Diabetes; hIAPP.

Publication types

Research Support, N.I.H., Extramural
Research Support, N.I.H., Intramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Amyloid / chemistry*
Amyloid beta-Peptides / chemistry*
Amyloid beta-Peptides / isolation & purification
Humans
Microscopy
Models, Molecular
Molecular Dynamics Simulation
Monte Carlo Method
Protein Multimerization
Spectrum Analysis

Substances

Amyloid
Amyloid beta-Peptides

Abstract

Publication types

MeSH terms

Substances

Grants and funding