In order to study the effects of salicylic acid on the growth and physiological performance of Ulva prolifera with different proliferative styles, we took U. prolifera from vegetative (VU) and spore proliferative cells (SU) as materials, and cultured them under different salicylic acid concentrations to investigate their growth rate, chlorophyll fluorescence, SOD and soluble protein content. The results showed that the growth of both VU and SU was promoted by low concentration of salicy-lic acid, especially for VU. Under 0.2 Μg·mL-1 salicylic acid treatment, VU showed the highest relative growth rate of 21.0%, and the maximum photochemical efficiency (Fv/Fm) increased by 9.8% compared with SU. Additionally, salicylic acid affected the SOD activity significantly, and the enzyme activity of VU increased by 52.0% and 198.6% under 0.2 and 0.5 Μg·mL- 1 salicylic acid treatment, and that of SU increased by 54.1% and 38.0%, respectively. Salicylic acid also promoted the relative electron transfer rate (rETR), photosynthesis and protein content of both VU and SU. In conclusion, salicylic acid benefited the growth of two kinds of U. prolifera, especially for VU.
为探讨水杨酸(SA)对不同增殖方式来源的浒苔生长和生理特性的影响,本文选取营养繁殖得到的浒苔(VU)和孢子/配子繁殖得到的浒苔(SU)作为供试材料,设置不同的水杨酸浓度,测定两种浒苔的生长、叶绿素荧光、超氧化物歧化酶(SOD)和可溶性蛋白含量等生理指标.结果表明: 低浓度水杨酸可以促进VU和SU的生长,对VU促进效果更为显著;在0.2 μg·mL-1水杨酸浓度下,VU相对生长速率达到最大值21.0%,且与SU相比,VU最大光化学效率提高了9.8%.水杨酸对两种浒苔的SOD活性影响较大,在水杨酸浓度为0.2、0.5 μg·mL-1下,VU的SOD活性增幅分别达52.0%、198.6%,SU的SOD活性增幅分别达54.1%、38.0%.水杨酸促进了浒苔的相对电子传递速率、光合作用和蛋白质含量.水杨酸对两种增殖方式来源的浒苔的生长均有促进作用,尤其是对VU的促进作用更为明显.
Keywords: Ulva prolifera; antioxidant; chlorophyll fluorescence; proliferation; salicylic acid.