Bioinspired, biocompatible and peptide-decorated silk fibroin coatings for enhanced osteogenesis of bioinert implant

J Biomater Sci Polym Ed. 2018 Sep;29(13):1595-1611. doi: 10.1080/09205063.2018.1477316. Epub 2018 May 23.

Abstract

In this study, we develop an osteopromotive polyetheretherketone (PEEK) implant decorated with silk fibroin and bone forming peptide, in which the surface of bioinert PEEK implant is firstly sulfonated to form a three-dimensional, porous topography and then is functionalized with silk fibroin via spin-coating process and peptide decoration. The bio-test results show that cells on the functional bioinert implants exhibit better cell adhesion, proliferation and spreading, when compared with the uncoated ones. Moreover, the peptide-decorated silk fibroin coatings have ability to hasten the osteogenic differentiation and maturation of osteoblast-like cells. Our findings show the potential of the functional PEEK implants with superior bioactivity and osteoinductive property in orthopedics and dentistry. Besides, the facile, bioinspired, osteopromotive modification strategy can be used in other orthopedic and dental implants, such as titanium, zirconium dioxide.

Keywords: Bioinert implant; PEEK; osteogenesis; peptide; porous; silk fibroin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Benzophenones
  • Cell Adhesion / drug effects
  • Cell Line
  • Cell Proliferation / drug effects
  • Cell Survival / drug effects
  • Coated Materials, Biocompatible / chemistry
  • Coated Materials, Biocompatible / metabolism
  • Fibroins / chemistry*
  • Humans
  • Ketones / chemistry
  • Ketones / metabolism
  • Osteoblasts / chemistry*
  • Osteogenesis
  • Peptides / chemistry*
  • Polyethylene Glycols / chemistry
  • Polyethylene Glycols / metabolism
  • Polymers
  • Porosity
  • Prostheses and Implants*
  • Silk / chemistry*
  • Surface Properties
  • Tissue Engineering / methods
  • Tissue Scaffolds / chemistry

Substances

  • Benzophenones
  • Coated Materials, Biocompatible
  • Ketones
  • Peptides
  • Polymers
  • Silk
  • polyetheretherketone
  • Polyethylene Glycols
  • Fibroins