Functional Studies on Steroid Receptors

Methods Mol Biol. 2018:1786:117-130. doi: 10.1007/978-1-4939-7845-8_7.

Abstract

Nuclear receptors play an important role in prostate cancer and the androgen receptor is a key transcription factor in regulation of cellular events. Androgen receptor-associated coregulators may be upregulated or downregulated in prostate cancer. Altered expression of regulators may potentiate androgen-induced proliferation, migration, and invasion. Therapies aimed to modulate the function of coregulators in prostate cancer may be based on the use of small molecule inhibitors. Expression and function of AR-associated proteins could be investigated after overexpression and gene silencing followed by hormonal treatment, real-time RT-PCR and ChIP.

Keywords: Androgen receptor; Coregulatory proteins; Prostate cancer progression; Transcriptional complexes.

MeSH terms

  • Disease Progression
  • Humans
  • Male
  • Multiprotein Complexes / metabolism
  • Prostatic Neoplasms / genetics
  • Prostatic Neoplasms / metabolism
  • Prostatic Neoplasms / pathology
  • Protein Binding
  • Real-Time Polymerase Chain Reaction
  • Receptors, Androgen / metabolism
  • Receptors, Steroid / chemistry
  • Receptors, Steroid / genetics*
  • Receptors, Steroid / metabolism*
  • Transcription Factors / metabolism
  • Transcription, Genetic

Substances

  • Multiprotein Complexes
  • Receptors, Androgen
  • Receptors, Steroid
  • Transcription Factors