Lectin (UEA-1) reaction of capillary endothelium with reference to permeability in autopsied cases of cerebral infarction

Histol Histopathol. 1986 Jul;1(3):219-26.

Abstract

The relationship between endothelial reactivity to Ulex europaeus agglutinin-1 (UEA-1) and the permeability of the vascular wall in human autopsied cases of cerebral infarction was studied. Sections from the cerebral cortex were reacted with horseradish peroxidase UEA-1 to demonstrate the surface membrane of endothelial cells. Albumin in the neuropil of sections was demonstrated for the estimation of increased vascular permeability. The results showed that endothelial reactivity to UEA-1 was reduced in cases where death had occurred 3 to 5 days after onset of cerebral infarction. Reactivity was also diminished in cases where death had occurred after 13 and 25 days; these cases showed fresh ischemic lesions caused by re-attacks of infarction. Albumin extravasation into the neuropil was demonstrated in these intermediate cases. Chronic cases, dying after more than 52 days, showed no reduction of endothelial reactivity to UEA-1 and no albumin extravasation was proved. It was concluded that UEA-1 can be employed as a useful morphological marker for evaluation of endothelial function and vascular permeability.

Publication types

  • Comparative Study

MeSH terms

  • Aged
  • Aged, 80 and over
  • Autopsy
  • Capillary Permeability
  • Cerebral Infarction / metabolism
  • Cerebral Infarction / pathology*
  • Endothelium, Vascular / metabolism*
  • Endothelium, Vascular / ultrastructure
  • Female
  • Histocytochemistry
  • Humans
  • Lectins*
  • Male
  • Plant Lectins*
  • Serum Albumin / analysis

Substances

  • Lectins
  • Plant Lectins
  • Serum Albumin
  • Ulex europaeus lectins