A probable prehistoric case of meningococcal disease from San Francisco Bay: Next generation sequencing of Neisseria meningitidis from dental calculus and osteological evidence

Int J Paleopathol. 2018 Sep:22:173-180. doi: 10.1016/j.ijpp.2018.05.001. Epub 2018 May 25.

Abstract

Next Generation Sequencing (NGS) of ancient dental calculus samples from a prehistoric site in San Francisco Bay, CA-SCL-919, reveals a wide range of potentially pathogenic bacteria. One older adult woman, in particular, had high levels of Neisseria meningitidis and low levels of Haemophilus influenzae, species that were not observed in the calculus from three other individuals. Combined with the presence of incipient endocranial lesions and pronounced meningeal grooves, we interpret this as an ancient case of meningococcal disease. This disease afflicts millions around the globe today, but little is known about its (pre)history. With additional sampling, we suggest NGS of calculus offers an exciting new window into the evolutionary history of these bacterial species and their interactions with humans.

Keywords: Dental calculus; Meningococcal disease; Neisseria meningitidis; Next generation sequencing.

Publication types

  • Historical Article
  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA, Bacterial / analysis
  • Dental Calculus / microbiology*
  • Female
  • High-Throughput Nucleotide Sequencing / methods*
  • History, Ancient
  • Humans
  • Meningitis, Meningococcal / history*
  • Middle Aged
  • Neisseria meningitidis
  • Paleopathology / methods*
  • San Francisco
  • Skull / pathology

Substances

  • DNA, Bacterial