Isolation and characterisation of a cDNA clone for human apolipoprotein CI and assignment of the gene to chromosome 19

F Tata; I Henry; A F Markham; S C Wallis; D Weil; K H Grzeschik; C Junien; R Williamson; S E Humphries

doi:10.1007/BF00291654

Isolation and characterisation of a cDNA clone for human apolipoprotein CI and assignment of the gene to chromosome 19

Hum Genet. 1985;69(4):345-9. doi: 10.1007/BF00291654.

Authors

F Tata, I Henry, A F Markham, S C Wallis, D Weil, K H Grzeschik, C Junien, R Williamson, S E Humphries

PMID: 2985493
DOI: 10.1007/BF00291654

Abstract

We have synthesised a mixed oligonucleotide 17 bases long and used it to isolate cDNA clones for apolipoprotein CI (apo CI) from an adult liver cDNA library. The partial sequence of one of these clones confirms its identity. We have used this probe and Southern blotting techniques to identify the human apo CI gene in DNA from a series of rodent X human somatic cell hybrids. Our results provide evidence for the assignment of this gene to human chromosome 19.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Apolipoprotein C-I
Apolipoproteins C / genetics*
Base Sequence
Cell Line
Chromosome Mapping*
Chromosomes, Human, 19-20*
Cloning, Molecular
Cricetinae
DNA / genetics
DNA / isolation & purification*
DNA Restriction Enzymes
DNA, Recombinant
Humans
Hybrid Cells
Mice
Oligonucleotides

Substances

Apolipoprotein C-I
Apolipoproteins C
DNA, Recombinant
Oligonucleotides
DNA
DNA Restriction Enzymes