Super high resolution-single molecule-sequence-based typing (SS-SBT) is an HLA DNA typing method to the field 4 level of allelic resolution (formerly known as 8-digit typing) to efficiently detect novel and null alleles without phase ambiguity by combination of long ranged PCR amplification and next-generation sequencing (NGS) technologies. In this chapter, we describe three basic steps, long ranged PCR, NGS, and allele assignment.
Keywords: Genotyping; HLA; Ion Torrent PGM; Next-generation sequencing (NGS); Super high resolution single molecule—sequence-based typing (SS-SBT); long ranged polymerase chain reaction (PCR).