Photo-Induced Electron Transfer Real-Time PCR for Detection of Plasmodium falciparum plasmepsin 2 Gene Copy Number

Antimicrob Agents Chemother. 2018 Jul 27;62(8):e00317-18. doi: 10.1128/AAC.00317-18. Print 2018 Aug.

Abstract

Piperaquine is an important partner drug used in artemisinin-based combination therapies (ACTs). An increase in the plasmepsin 2 and 3 gene copy numbers has been associated with decreased susceptibility of Plasmodium falciparum to piperaquine in Cambodia. Here, we developed a photo-induced electron transfer real-time PCR (PET-PCR) assay to quantify the copy number of the P. falciparumplasmepsin 2 gene (PfPM2) that can be used in countries where P. falciparum is endemic to enhance molecular surveillance.

Keywords: PET-PCR; copy number; malaria; piperaquine.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antimalarials / chemistry
  • Antimalarials / pharmacology*
  • Aspartic Acid Endopeptidases / genetics*
  • Aspartic Acid Endopeptidases / metabolism
  • DNA Primers / chemical synthesis
  • DNA Primers / metabolism
  • Drug Resistance / genetics
  • Electron Transport
  • Gene Dosage*
  • Gene Expression
  • Light
  • Photochemical Processes
  • Plasmodium falciparum / drug effects
  • Plasmodium falciparum / genetics*
  • Plasmodium falciparum / growth & development
  • Protozoan Proteins / genetics*
  • Protozoan Proteins / metabolism
  • Quinolines / chemistry
  • Quinolines / pharmacology*
  • Real-Time Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity

Substances

  • Antimalarials
  • DNA Primers
  • Protozoan Proteins
  • Quinolines
  • piperaquine
  • Aspartic Acid Endopeptidases
  • plasmepsin II