Extracellular ATP and IL-23 Form a Local Inflammatory Circuit Leading to the Development of a Neutrophil-Dependent Psoriasiform Dermatitis

J Invest Dermatol. 2018 Dec;138(12):2595-2605. doi: 10.1016/j.jid.2018.05.018. Epub 2018 Jun 2.

Abstract

Psoriasis is a chronic inflammatory skin disease dependent on the IL-23/IL-17 axis, a potent inflammatory pathway involved in pathogen clearance and autoimmunity. Several triggers have been proposed as initiators for psoriasis, including alarmins such as adenosine triphosphate. However, the role of alarmins in psoriasis pathogenesis and cutaneous inflammation has not been well addressed. Studies show that signaling through the P2X7 receptor (P2X7R) pathway underlies the development of psoriasiform inflammation. In this regard, psoriasiform dermatitis induced by IL-23 is dependent on P2X7R signaling. Furthermore, direct activation of the P2X7R is sufficient to induce a well-characterized psoriasiform dermatitis. Mechanistic studies determined that P2X7R-induced inflammation is largely dependent on the IL-1β/NLRP3 inflammasome pathway and neutrophils. In conclusion, this work provides basic mechanistic insight into local inflammatory circuits induced after purinergic P2X7R signaling that are likely involved in the pathogenesis of many inflammatory diseases, such as psoriasis.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adenosine Triphosphate / metabolism
  • Animals
  • Dermatitis / immunology*
  • Disease Models, Animal
  • Extracellular Space / metabolism
  • Female
  • Humans
  • Imiquimod
  • Inflammation Mediators / metabolism
  • Interleukin-17 / metabolism
  • Interleukin-1beta / metabolism
  • Interleukin-23 / genetics
  • Interleukin-23 / metabolism*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • NLR Family, Pyrin Domain-Containing 3 Protein / metabolism
  • Neutrophils / immunology*
  • Psoriasis / chemically induced
  • Psoriasis / immunology*
  • Receptors, Purinergic P2X7 / genetics
  • Receptors, Purinergic P2X7 / metabolism*
  • Signal Transduction
  • Skin / pathology*

Substances

  • Inflammation Mediators
  • Interleukin-17
  • Interleukin-1beta
  • Interleukin-23
  • NLR Family, Pyrin Domain-Containing 3 Protein
  • Receptors, Purinergic P2X7
  • Adenosine Triphosphate
  • Imiquimod