Abstract
A new 5'-nucleotidase inhibitor named nucleoticidin was produced by Pseudomonas sp. YM-3229G. It was isolated from a fermentation broth by trichloroacetic acid extraction, ethanol precipitation and Dowex 1 and DEAE-52 column chromatography. It inhibited 5'-nucleotidase activity of snake venom and rat liver membrane. It also showed antitumor activity against solid type Sarcoma 180.
MeSH terms
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Animals
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Anti-Bacterial Agents / isolation & purification*
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Anti-Bacterial Agents / pharmacology
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Antibiotics, Antineoplastic / isolation & purification
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Antibiotics, Antineoplastic / pharmacology
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Cell Survival / drug effects
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Fermentation
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Liver / enzymology
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Nucleotidases / antagonists & inhibitors*
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Pseudomonas / metabolism
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Rats
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Sarcoma, Experimental / metabolism
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Snake Venoms / antagonists & inhibitors
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Soil Microbiology
Substances
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Anti-Bacterial Agents
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Antibiotics, Antineoplastic
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Snake Venoms
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Nucleotidases
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nucleotidase