C11-oxy C19 and C11-oxy C21 steroids in neonates: UPC2-MS/MS quantification of plasma 11β-hydroxyandrostenedione, 11-ketotestosterone and 11-ketoprogesterone

Steroids. 2018 Oct:138:1-5. doi: 10.1016/j.steroids.2018.06.003. Epub 2018 Jun 6.

Abstract

The purpose of this study was to identify the C11-oxy C19 and C11-oxy C21 steroids in male and female neonate plasma. At birth, the most abundant C11-oxy steroids detected in neonatal plasma were 11β-hydroxyandrostenedione, ∼13 nM, and 11-ketoprogesterone, ∼23 nM. C11-oxy C19 steroids were higher than C19 steroids in neonatal plasma, 22.2 nM vs 5.4 nM. The inclusion of C11-oxy C19 and C21 steroid reference ranges in routine steroid analyses will assist the characterization of disorders associated with impaired steroidogenic enzyme expression and the identification of potential biomarkers.

Keywords: 11keto-dihydrotestosterone (11KDHT); 11β-hydroxysteroid dehydrogenase (11βHSD); Congenital adrenal hyperplasia (CAH); Congenital hypogonadotropic hypogonadism (CHH); Disorders of sexual development (DSD); Newborn care.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Androstenols / blood*
  • Female
  • Humans
  • Infant, Newborn
  • Male
  • Progesterone / analogs & derivatives*
  • Progesterone / blood
  • Steroids / blood*
  • Tandem Mass Spectrometry / methods*
  • Testosterone / analogs & derivatives*
  • Testosterone / blood

Substances

  • Androstenols
  • Steroids
  • Testosterone
  • Progesterone
  • 11-ketoprogesterone
  • 11-ketotestosterone