Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1-18, and development of two multiplex PCRs for comprehensive capsule typing

Vet Microbiol. 2018 Jul:220:83-89. doi: 10.1016/j.vetmic.2018.05.011. Epub 2018 May 20.

Abstract

Problems with serological cross-reactivity have led to development of a number of PCRs (individual and multiplex) for molecular typing of Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia. Most of these assays were developed for detection of specific amplicons within capsule biosynthetic genes before the availability of complete sequences for the different serovars. Here we describe comparative analysis of the complete capsular loci for all 18 serovars of A. pleuropneumoniae, and development of two multiplex PCRs for comprehensive capsule typing of this important pig pathogen.

Keywords: A. pleuropneumoniae; Capsule typing; Diagnostic; Serovars; mPCR.

Publication types

  • Comparative Study

MeSH terms

  • Actinobacillus Infections / diagnosis
  • Actinobacillus Infections / microbiology
  • Actinobacillus pleuropneumoniae / classification
  • Actinobacillus pleuropneumoniae / genetics*
  • Actinobacillus pleuropneumoniae / pathogenicity
  • Animals
  • Bacterial Capsules / chemistry
  • Bacterial Capsules / classification*
  • Bacterial Capsules / genetics*
  • Multiplex Polymerase Chain Reaction / methods*
  • Polysaccharides, Bacterial / genetics*
  • Sequence Analysis*
  • Serogroup
  • Serotyping
  • Swine
  • Swine Diseases / diagnosis*
  • Swine Diseases / microbiology

Substances

  • Polysaccharides, Bacterial