LAIR-1 activation inhibits inflammatory macrophage phenotype in vitro

Cell Immunol. 2018 Sep:331:78-84. doi: 10.1016/j.cellimm.2018.05.011. Epub 2018 May 31.

Abstract

Macrophages are key cell types of innate immunity and play a central role in inflammation and host defense. Leukocyte-associated Ig-like receptor-1 (LAIR-1) is highly expressed on macrophages and regulates macrophage functions in several conditions. However, whether LAIR-1 is involved in governing macrophage polarization is still not clear. Here, we investigated the effect of LAIR-1 on macrophage polarization using human macrophage polarization model with THP-1 cells. It was found that LAIR-1 was highly expressed in THP-1 macrophages. IFN-γ reduced LAIR-1 expression in THP-1 macrophages. However, IL-4 did not have an effect on the expression of LAIR-1. Moreover, activation of LAIR-1 significantly inhibited the proinflammatory M1-like macrophage differentiation and promoted alternative activation of macrophages. Therefore, LAIR-1 may play critical roles in macrophage polarization. This study provides a rationale for macrophage polarization and sheds light on homeostatic mechanism in which LAIR-1 activation can terminate inflammation which may be impaired in patients with autoimmune disease.

Keywords: ITIM; Inflammation; LAIR-1; Macrophage polarization.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Differentiation / genetics
  • Cell Differentiation / immunology*
  • Cells, Cultured
  • Gene Expression / drug effects
  • Humans
  • Interferon-gamma / pharmacology
  • Lipopolysaccharides / pharmacology
  • Macrophage Activation / genetics
  • Macrophage Activation / immunology*
  • Macrophages / cytology
  • Macrophages / immunology*
  • Macrophages / metabolism
  • Phenotype
  • Receptors, Immunologic / genetics
  • Receptors, Immunologic / immunology*
  • Receptors, Immunologic / metabolism
  • THP-1 Cells
  • U937 Cells

Substances

  • Lipopolysaccharides
  • Receptors, Immunologic
  • leukocyte-associated immunoglobulin-like receptor 1
  • Interferon-gamma