GPU-based deep convolutional neural network for tomographic phase microscopy with ℓ1 fitting and regularization

J Biomed Opt. 2018 Jun;23(6):1-7. doi: 10.1117/1.JBO.23.6.066003.

Abstract

Tomographic phase microscopy (TPM) is a unique imaging modality to measure the three-dimensional refractive index distribution of transparent and semitransparent samples. However, the requirement of the dense sampling in a large range of incident angles restricts its temporal resolution and prevents its application in dynamic scenes. Here, we propose a graphics processing unit-based implementation of a deep convolutional neural network to improve the performance of phase tomography, especially with much fewer incident angles. As a loss function for the regularized TPM, the ℓ1-norm sparsity constraint is introduced for both data-fidelity term and gradient-domain regularizer in the multislice beam propagation model. We compare our method with several state-of-the-art algorithms and obtain at least 14 dB improvement in signal-to-noise ratio. Experimental results on HeLa cells are also shown with different levels of data reduction.

Keywords: GPU-based implementation; neural network; refractive index; tomographic phase microscopy; ℓ1 data-fidelity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms
  • Cell Count
  • HeLa Cells / cytology*
  • Humans
  • Image Processing, Computer-Assisted*
  • Imaging, Three-Dimensional
  • Microscopy, Phase-Contrast / instrumentation*
  • Neural Networks, Computer*
  • Signal-To-Noise Ratio
  • Tomography