Abstract
Vacuolar trafficking plays a vital role in plant growth and development. In this chapter, we describe a powerful technique for the evaluation of vacuolar protein trafficking, which is designated as GREEN FLUORESCENT SEED. Based on vacuole-targeted green fluorescent protein in Arabidopsis seeds, this method enables the nondestructive isolation of mutant seeds defective in vacuolar trafficking and their visual characterization.
Keywords:
Arabidopsis thaliana; GREEN FLUORESCENT SEED (GFS); Green fluorescent protein (GFP); Vacuolar sorting receptor 1 (VSR1); Vacuolar trafficking; Vacuole.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Arabidopsis / genetics
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Arabidopsis / metabolism*
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / metabolism*
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism*
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Microscopy, Confocal / methods
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Microscopy, Fluorescence / methods
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Mutagenesis
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Protein Transport
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Seeds / genetics
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Seeds / metabolism*
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Vacuoles / genetics
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Vacuoles / metabolism*
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Vesicular Transport Proteins / genetics
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Vesicular Transport Proteins / metabolism*
Substances
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Arabidopsis Proteins
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Vesicular Transport Proteins
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Green Fluorescent Proteins