A new cause of false positive voriconazole levels: Watch your collection tubes!

Almudena Burillo; Alicia Gómez-López; Pilar Escribano; Alicia Galar; Beatriz Salinas; Nadire Díaz-Pérez; Patricia Muñoz; Emilio Bouza

doi:10.1016/j.jchromb.2018.06.022

A new cause of false positive voriconazole levels: Watch your collection tubes!

J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Aug 15:1092:328-331. doi: 10.1016/j.jchromb.2018.06.022. Epub 2018 Jun 13.

Authors

Almudena Burillo¹, Alicia Gómez-López², Pilar Escribano³, Alicia Galar³, Beatriz Salinas⁴, Nadire Díaz-Pérez⁵, Patricia Muñoz⁶, Emilio Bouza⁷

Affiliations

¹ Clinical Microbiology and Infectious Diseases Department, Hospital General Universitario Gregorio Marañón, Doctor Esquerdo 46, 28007 Madrid, Spain; Instituto de Investigación Sanitaria, Hospital Gregorio Marañón, Doctor Esquerdo 46, 28007 Madrid, Spain; Department of Medicine, School of Medicine, Universidad Complutense de Madrid, Avda. Complutense s/n, 28040 Madrid, Spain. Electronic address: [email protected].
² Laboratory of Reference and Research on Mycology, National Center for Microbiology, Instituto de Salud Carlos III, Carretera de Majadahonda-Pozuelo Km. 2.200, 28220 Majadahonda, Spain. Electronic address: [email protected].
³ Instituto de Investigación Sanitaria, Hospital Gregorio Marañón, Doctor Esquerdo 46, 28007 Madrid, Spain.
⁴ Instituto de Investigación Sanitaria, Hospital Gregorio Marañón, Doctor Esquerdo 46, 28007 Madrid, Spain; Advanced Imaging Unit, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Melchor Fernández Almagro 3, 28029 Madrid, Spain. Electronic address: [email protected].
⁵ Clinical Microbiology and Infectious Diseases Department, Hospital General Universitario Gregorio Marañón, Doctor Esquerdo 46, 28007 Madrid, Spain; Instituto de Investigación Sanitaria, Hospital Gregorio Marañón, Doctor Esquerdo 46, 28007 Madrid, Spain.
⁶ Clinical Microbiology and Infectious Diseases Department, Hospital General Universitario Gregorio Marañón, Doctor Esquerdo 46, 28007 Madrid, Spain; Instituto de Investigación Sanitaria, Hospital Gregorio Marañón, Doctor Esquerdo 46, 28007 Madrid, Spain; Department of Medicine, School of Medicine, Universidad Complutense de Madrid, Avda. Complutense s/n, 28040 Madrid, Spain; CIBER Enfermedades Respiratorias-CIBERES (CB06/06/0058), Doctor Esquerdo 46, 28007 Madrid, Spain. Electronic address: [email protected].
⁷ Clinical Microbiology and Infectious Diseases Department, Hospital General Universitario Gregorio Marañón, Doctor Esquerdo 46, 28007 Madrid, Spain; Instituto de Investigación Sanitaria, Hospital Gregorio Marañón, Doctor Esquerdo 46, 28007 Madrid, Spain; Department of Medicine, School of Medicine, Universidad Complutense de Madrid, Avda. Complutense s/n, 28040 Madrid, Spain; CIBER Enfermedades Respiratorias-CIBERES (CB06/06/0058), Doctor Esquerdo 46, 28007 Madrid, Spain.

PMID: 29936368
DOI: 10.1016/j.jchromb.2018.06.022

Abstract

We communicate the interference of a compound of the blood collection tube with the accuracy of a validated high-pressure liquid chromatography method with ultraviolet detection for quantifying voriconazole levels, which led to false positive results. This could have serious consequences for patient management. Our advice is to implement external assessment programs.

Keywords: Chromatography; Clinical laboratory techniques; Data accuracy; High-pressure liquid; Therapeutic drug monitoring; Voriconazole.

MeSH terms

Adult
Antifungal Agents / blood*
Blood Chemical Analysis / standards*
Blood Specimen Collection* / instrumentation
Blood Specimen Collection* / standards
Child
Chromatography, High Pressure Liquid
Drug Monitoring / standards*
False Positive Reactions*
Humans
Voriconazole / blood*

Substances

Antifungal Agents
Voriconazole