Abstract
A method is described for the isolation and purification of non-synaptic and synaptic mitochondria and plasma membranes. The procedure involved differential centrifugation and successively a Ficoll gradient followed by a Percoll one. The method is rapid and maintains constant osmotic conditions. The Percoll-purified fractions show a lower density than those prepared by the conventional methods. The fractions were identified and characterized by enzyme markers and electron microscopy. The method will be useful for routine preparations rendering a good degree of homogeneity and purity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acid Phosphatase / analysis
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Animals
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Brain Chemistry
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Cell Fractionation / methods*
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Cell Membrane / enzymology
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Centrifugation, Density Gradient / methods
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Mitochondria / enzymology*
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Mitochondria / metabolism
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NADPH-Ferrihemoprotein Reductase / analysis
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Povidone
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Rats
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Rats, Inbred Strains
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Silicon Dioxide
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Sodium-Potassium-Exchanging ATPase / analysis
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Subcellular Fractions / ultrastructure
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Synaptosomes / enzymology*
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Synaptosomes / metabolism
Substances
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Percoll
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Silicon Dioxide
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NADPH-Ferrihemoprotein Reductase
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Acid Phosphatase
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Sodium-Potassium-Exchanging ATPase
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Povidone