In vivo multiphoton kinetic imaging of the toxic effect of carbon tetrachloride on hepatobiliary metabolism

Chih-Ju Lin; Sheng-Lin Lee; Hsuan-Shu Lee; Chen-Yuan Dong

doi:10.1117/1.JBO.23.9.091407

In vivo multiphoton kinetic imaging of the toxic effect of carbon tetrachloride on hepatobiliary metabolism

J Biomed Opt. 2018 Jun;23(9):1-6. doi: 10.1117/1.JBO.23.9.091407.

Authors

Chih-Ju Lin¹, Sheng-Lin Lee¹, Hsuan-Shu Lee², Chen-Yuan Dong^{1

3}

Affiliations

¹ National Taiwan University, Department of Physics, Taipei, Taiwan.
² National Taiwan University Hospital, Department of Internal Medicine, Taipei, Taiwan.
³ National Taiwan University, Molecular Imaging Center, Taipei, Taiwan.

PMID: 29943526
DOI: 10.1117/1.JBO.23.9.091407

Abstract

We used intravital multiphoton microscopy to study the recovery of hepatobiliary metabolism following carbon tetrachloride (CCl4) induced hepatotoxicity in mice. The acquired images were processed by a first order kinetic model to generate rate constant resolved images of the mouse liver. We found that with progression of hepatotoxicity, the spatial gradient of hepatic function disappeared. A CCl4-induced damage mechanism involves the compromise of membrane functions, resulting in accumulation of processed 6-carboxyfluorescein molecules. At day 14 following induction, a restoration of the mouse hepatobiliary function was found. Our approach allows the study of the response of hepatic functions to chemical agents in real time and is useful for studying pharmacokinetics of drug molecules through optical microscopic imaging.

Keywords: intravital multiphoton; journals; optics; photonics; templates.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Carbon Tetrachloride / toxicity*
Intravital Microscopy / methods*
Kinetics
Liver* / cytology
Liver* / drug effects
Liver* / metabolism
Male
Mice
Mice, Inbred C57BL
Microscopy, Fluorescence, Multiphoton / methods*
Time-Lapse Imaging

Substances

Carbon Tetrachloride