Targeting Detection and Inhibition of Methicillin-Resistant Staphylococcus aureus Pneumonia with a Theranostic Mesoporous Silica-Based Platform

J Biomed Nanotechnol. 2018 Jul 1;14(7):1298-1307. doi: 10.1166/jbn.2018.2580.

Abstract

In this study, non-invasive early stage diagnosis of methicillin-resistant Staphylococcus aureus-related pneumonia and related anti-inflammatory profiles with theranostic platforms were reported. Mesoporous silica NPs were first synthesized and chemically modified to bear an amino group and further covalently bind to amino-2,4,6-triiodoisophthalic acid and anti-methicillin-resistant S. aureus antibodies. The drug-loaded NPs were characterized by transmission electron microscopy, dynamic light scattering analysis and X-ray photoelectron spectra. MTT and broth dilution assays were used to investigate the biocompatibility and antibacterial potency of the platform. In vivo computed tomography analysis was performed in rats infected with different kinds of pneumonia, and anti-inflammatory studies such as bronchoalveolar lavage and pathological assays were used to test the capability of our nanomedicine as a theranostic platform. In addition, the long-term safety of the hybrid NPs was investigated via hematological and biochemical assays.

MeSH terms

  • Animals
  • Anti-Bacterial Agents
  • Methicillin
  • Methicillin-Resistant Staphylococcus aureus*
  • Pneumonia*
  • Porosity
  • Rats
  • Silicon Dioxide
  • Theranostic Nanomedicine

Substances

  • Anti-Bacterial Agents
  • Silicon Dioxide
  • Methicillin