Illuminating GPCR Signaling by Cryo-EM

Haaris Ahsan Safdari; Shubhi Pandey; Arun K Shukla; Somnath Dutta

doi:10.1016/j.tcb.2018.06.002

Illuminating GPCR Signaling by Cryo-EM

Trends Cell Biol. 2018 Aug;28(8):591-594. doi: 10.1016/j.tcb.2018.06.002. Epub 2018 Jun 23.

Authors

Haaris Ahsan Safdari¹, Shubhi Pandey², Arun K Shukla³, Somnath Dutta⁴

Affiliations

¹ Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560012, India.
² Department of Biological Sciences and Bioengineering, Indian Institute of Technology, Kanpur 208016, India.
³ Department of Biological Sciences and Bioengineering, Indian Institute of Technology, Kanpur 208016, India. Electronic address: [email protected].
⁴ Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560012, India. Electronic address: [email protected].

PMID: 29945844
DOI: 10.1016/j.tcb.2018.06.002

Abstract

The wave of resolution revolution in cryo-EM has touched, and made a significant impact on, the structural biology of GPCRs. High-resolution structures of several GPCR-G-protein complexes are now determined by cryo-EM and they illuminate fine structural details of this central macromolecular complex involved in cellular signaling.

Keywords: GPCR; arrestins; biased signaling; cryo-EM; negative-staining.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Cryoelectron Microscopy
Humans
Receptors, G-Protein-Coupled / chemistry
Receptors, G-Protein-Coupled / metabolism*
Signal Transduction*

Substances

Receptors, G-Protein-Coupled

Grants and funding

Wellcome Trust/United Kingdom