How to create state-of-the-art genetic model systems: strategies for optimal CRISPR-mediated genome editing

Yannik Bollen; Jasmin Post; Bon-Kyoung Koo; Hugo J G Snippert

doi:10.1093/nar/gky571

How to create state-of-the-art genetic model systems: strategies for optimal CRISPR-mediated genome editing

Nucleic Acids Res. 2018 Jul 27;46(13):6435-6454. doi: 10.1093/nar/gky571.

Authors

Yannik Bollen^{1

2

3}, Jasmin Post^{1

2}, Bon-Kyoung Koo⁴, Hugo J G Snippert^{1

2}

Affiliations

¹ Molecular Cancer Research, Center for Molecular Medicine, University Medical Center Utrecht, Utrecht University, The Netherlands.
² Oncode Institute, The Netherlands.
³ Medical Cell BioPhysics, MIRA Institute, University of Twente, Enschede, The Netherlands.
⁴ Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna, Austria.

Abstract

Model systems with defined genetic modifications are powerful tools for basic research and translational disease modelling. Fortunately, generating state-of-the-art genetic model systems is becoming more accessible to non-geneticists due to advances in genome editing technologies. As a consequence, solely relying on (transient) overexpression of (mutant) effector proteins is no longer recommended since scientific standards increasingly demand genetic modification of endogenous loci. In this review, we provide up-to-date guidelines with respect to homology-directed repair (HDR)-mediated editing of mammalian model systems, aimed at assisting researchers in designing an efficient genome editing strategy.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

CRISPR-Associated Protein 9
CRISPR-Cas Systems*
Endodeoxyribonucleases
Gene Editing*
Models, Genetic*
Polymerase Chain Reaction
Recombinational DNA Repair

Substances

CRISPR-Associated Protein 9
Endodeoxyribonucleases