Abstract
The generation of allergen-specific TCR transgenic animals allows for the characterization of allergen-specific T-cell responses in vivo and in vitro and is a powerful tool to study adaptive immunity to allergens. Here we describe an approach starting from the isolation of antigen-specific T-cell hybridomas and using PCR, flow cytometric, and co-culture methods to obtain antigen-specific MHC class II-restricted CD4+ TCR transgenic mice on the Rag2-/- background.
Keywords:
Allergen; Hybridoma; T-cell fusion; T-cell receptor; Transgenic.
MeSH terms
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Allergens / immunology*
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Animals
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CD4-Positive T-Lymphocytes / immunology
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CD4-Positive T-Lymphocytes / metabolism
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Cell Communication / immunology
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Cell Line
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Cloning, Molecular
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Dendritic Cells / immunology
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Dendritic Cells / metabolism
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Gene Expression
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Gene Order
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Genetic Vectors / genetics
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Lymph Nodes / innervation
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Lymph Nodes / metabolism
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Mice
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Mice, Transgenic
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Receptors, Antigen, T-Cell / genetics
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Receptors, Antigen, T-Cell / metabolism*
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T-Cell Antigen Receptor Specificity / genetics
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T-Cell Antigen Receptor Specificity / immunology*
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T-Lymphocyte Subsets / immunology*
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T-Lymphocyte Subsets / metabolism*
Substances
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Allergens
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Receptors, Antigen, T-Cell