[Evaluation of the size of the continuous poly(G) site necessary for the biological activity of the poly(G).poly(C) complex]

Vopr Virusol. 1985 May-Jun;30(3):337-40.
[Article in Russian]

Abstract

On the basis of synthesis of a series of poly(G, A).poly(C) copolymers with changing G:A ratio from 15:1 to 90:1 and trials of their biological activity in comparison with poly(G).poly(C), the size of poly(G) in it was evaluated within the range of a continuous double-stranded area necessary for the activity. The antiviral activity close to that of poly(G).poly(C) in experimental tick-borne encephalitis of mice and vesicular stomatitis virus infection of chick embryo cells was found only in poly(G,A).poly(C) complexes with a G:A ratio equal to or higher than 90:1. Consequently, the high activity of poly(G).poly(C) is present at an average length of poly(G) equal to 90-100 nucleotides within the limits of the continuous double-stranded area.

Publication types

  • Comparative Study
  • English Abstract

MeSH terms

  • Animals
  • Base Sequence
  • Chick Embryo
  • Drug Evaluation, Preclinical
  • Encephalitis, Tick-Borne / drug therapy
  • Mice
  • Mice, Inbred BALB C
  • Molecular Weight
  • Poly C / analysis
  • Poly C / therapeutic use*
  • Poly G / analysis
  • Poly G / therapeutic use*
  • Polyribonucleotides / analysis
  • Polyribonucleotides / therapeutic use*
  • Structure-Activity Relationship
  • Vesicular stomatitis Indiana virus / drug effects
  • Viral Plaque Assay

Substances

  • Polyribonucleotides
  • Poly G
  • poly G-poly C
  • Poly C
  • poly(G,A).poly(C)