The influence of sigma factors and ribosomal recognition elements on heterologous expression of cyanobacterial gene clusters in Escherichia coli

FEMS Microbiol Lett. 2018 Aug 1;365(15):fny164. doi: 10.1093/femsle/fny164.

Abstract

Cyanobacterial natural products offer new possibilities for drugs and lead compounds but many factors can inhibit the production of sufficient yields for pharmaceutical processes. While Escherichia coli and Streptomyces sp. have been used as heterologous expression hosts to produce cyanobacterial natural products, they have not met with resounding success largely due to their inability to recognize cyanobacterial promoter regions. Recent work has shown that the filamentous freshwater cyanobacterium Anabaena sp. strain PCC 7120 recognizes various cyanobacterial promoter regions and can produce lyngbyatoxin A from the native promoter. Introduction of Anabaena sigma factors into E. coli might allow the native transcriptional machinery to recognize cyanobacterial promoters. Here, all 12 Anabaena sigma factors were expressed in E. coli and subsets were found to initiate transcription from several cyanobacterial promoters based on transcriptional fusions to the chloramphenicol acetyltransferase (CAT) reporter. Expression of individual Anabaena sigma factors in E. coli did not result in lyngbyatoxin A production from its native cyanobacterial gene cluster, possibly hindered by deficiencies in recognition of cyanobacterial ribosomal binding sites by native E. coli translational machinery. This represents an important step toward engineering E. coli into a general heterologous expression host for cyanobacterial biosynthetic gene cluster expression.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anabaena / genetics*
  • Anabaena / metabolism
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • Escherichia coli / genetics*
  • Escherichia coli / metabolism
  • Gene Expression*
  • Lyngbya Toxins / metabolism
  • Multigene Family
  • Peptide Chain Initiation, Translational
  • Promoter Regions, Genetic
  • Ribosomes / genetics
  • Ribosomes / metabolism*
  • Sigma Factor / genetics*
  • Sigma Factor / metabolism

Substances

  • Bacterial Proteins
  • Lyngbya Toxins
  • Sigma Factor
  • lyngbyatoxin A