Establishment of a novel human megakaryoblastic leukemia cell line, MEG-01, with positive Philadelphia chromosome

Blood. 1985 Dec;66(6):1384-92.

Abstract

A megakaryoblastic cell line, designated MEG-01, was established from the bone marrow of a patient with blast crisis of Philadelphia (Ph1) chromosome-positive chronic myelogenous leukemia. MEG-01 cells grew in single-cell suspension with a doubling time of 36 to 48 hours. Under the usual culture conditions, approximately half of the cells adhered to the culture flask with extention of pseudopods. MEG-01 cells were positive for the periodic acid-Schiff reaction, alpha-naphthyl acetate esterase, and acid phosphatase, and negative for myeloperoxidase, alpha-naphthyl butyrate esterase, naphthol AS-D chloroacetate esterase, and alkaline phosphatase. Ultrastructural platelet peroxidase was positive in MEG-01 cells. Cytoplasmic factor VIII (FVIII)-related antigen was weakly positive in larger MEG-01 cells by both an indirect immunofluorescent technique with monoclonal antibodies and a direct immunoperoxidase technique using horseradish peroxidase-conjugated conventional rabbit anti-human FVIII antibody. Platelet glycoprotein (GP) IIb/IIIa antigen was uniformly demonstrated on the surface of MEG-01 cells by both indirect immunofluorescent and immunoperoxidase techniques using antiplatelet GP IIb/IIIa monoclonal antibodies; platelet GP lb antigen was demonstrated only in the cytoplasm of larger MEG-01 cells. MEG-01 cells possessed no markers for B or T lymphocytes or for myeloid cells. Chromosome analysis of this cell line revealed a human male hyperdiploid karyotype with a modal chromosome number of 56 to 58. The Ph1 chromosome was observed in all karyotypes analyzed. This novel human megakaryoblastic cell line may provide a useful model for the study of human megakaryopoiesis and of the biosynthetic mechanisms of proteins unique to megakaryocytic lineage.

Publication types

  • Case Reports

MeSH terms

  • Antibodies, Monoclonal / immunology
  • Antigens, Viral / analysis
  • Blood Platelets / enzymology
  • Cell Line*
  • Cells, Cultured
  • DNA Nucleotidylexotransferase / metabolism
  • Herpesvirus 4, Human / immunology
  • Humans
  • Karyotyping
  • Leukemia, Myeloid / blood
  • Leukemia, Myeloid / pathology*
  • Male
  • Megakaryocytes / cytology*
  • Megakaryocytes / ultrastructure
  • Middle Aged
  • Muramidase / analysis
  • Neoplastic Stem Cells / cytology*
  • Peroxidases / metabolism
  • Phagocytosis
  • Rosette Formation

Substances

  • Antibodies, Monoclonal
  • Antigens, Viral
  • Peroxidases
  • DNA Nucleotidylexotransferase
  • Muramidase