Biofilms of Candida albicans and Streptococcus sanguinis and their susceptibility to antimicrobial effects of photodynamic inactivation

Ana Luiza do Rosário Palma; Lucas de Paula-Ramos; Nádia Domingues; Graziella Nuernberg Back-Brito; Luciane Dias de Oliveira; Cristiane Aparecida Pereira; Antonio Olavo Cardoso Jorge

doi:10.1016/j.pdpdt.2018.07.003

Biofilms of Candida albicans and Streptococcus sanguinis and their susceptibility to antimicrobial effects of photodynamic inactivation

Photodiagnosis Photodyn Ther. 2018 Dec:24:95-101. doi: 10.1016/j.pdpdt.2018.07.003. Epub 2018 Jul 7.

Authors

Ana Luiza do Rosário Palma¹, Lucas de Paula-Ramos², Nádia Domingues³, Graziella Nuernberg Back-Brito⁴, Luciane Dias de Oliveira⁵, Cristiane Aparecida Pereira⁶, Antonio Olavo Cardoso Jorge⁷

Affiliations

¹ São Paulo State University (Unesp), Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo, Brazil. Electronic address: [email protected].
² São Paulo State University (Unesp), Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo, Brazil. Electronic address: [email protected].
³ São Paulo State University (Unesp), Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo, Brazil. Electronic address: [email protected].
⁴ São Paulo State University (Unesp), Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo, Brazil. Electronic address: [email protected].
⁵ São Paulo State University (Unesp), Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo, Brazil. Electronic address: [email protected].
⁶ São Paulo State University (Unesp), Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo, Brazil. Electronic address: [email protected].
⁷ São Paulo State University (Unesp), Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo, Brazil. Electronic address: [email protected].

PMID: 29990641
DOI: 10.1016/j.pdpdt.2018.07.003

Abstract

This study evaluated the effects of photodynamic inactivation (PDI) on single and multi-species biofilms, compounds by Candida albicans and Streptococcus sanguinis. Biofilms were formed, on microplate of 96 wells, by suspensions of C. albicans (ATCC 18804) and S. sanguinis (ATCC 7073) adjusted in 10⁷ cells/mL, followed by incubation of 48 h (with 5% CO₂). The effects of the photosensitizer erythrosine (ER) at 400 μM for 5 min and green light-emitting diode (LED - 532 ± 10 nm) for 3 min, alone and conjugated, were evaluated. After normality test, results was analysed by Tukey´s test (P < 0.05). PDI group promoted reductions of 1.07 and 0.39 log₁₀, respectively, in biofilms of C. albicans alone and in association with S. sanguinis. Biofilms of S. sanguinis alone were more sensitive, with reduction of 4.48 log₁₀. When in association with the yeast, S. sanguinis have a decrease of 2.67 log₁₀. SEM analysis revealed a decrease in bacterial and fungal structures of biofilms treated with PDI. In conclusion PDI promoted significant microbial reductions in both species of microorganisms grown on mixed biofilms. This study is one of the pioneers to evaluate the antimicrobial action of PDI on biofilms of S. sanguinis and C. albicans, demonstrating a way to control these microorganisms of clinical importance.

Keywords: Antimicrobial, Antimicrobial -Photodynamic Therapy; Biofilm; Candida albicans; Photodynamic inactivation; Streptococcus sanguinis.

MeSH terms

Biofilms / drug effects
Candida albicans / drug effects*
Erythrosine / pharmacology*
Photochemotherapy / methods*
Photosensitizing Agents / pharmacology*
Streptococcus sanguis / drug effects*

Substances

Photosensitizing Agents
Erythrosine