Commercial personal care product mixtures exhibit hormetic concentration-responses to Vibrio qinghaiensis sp.-Q67

The biological effects related to personal care products (PCPs) are almost induced by some active ingredients in the PCPs rather than the PCP itself. In this study, 23 common and widely used toner, skin water, and make-up water (TSM) commodities were directly taken as mixture samples, and Vibrio qinghaiensis sp.-Q67 (Q67) was used as the test organism. The toxicities of the TSMs to Q67 were determined via microplate toxicity analysis at 0.25 h and 12 h. Each TSM commodity can be regarded as a complicated mixture (relative concentration is 1). It was shown that the concentration-response curves (CRCs) of 23 TSMs are monotonic sigmoid-shaped (S-shaped) at 0.25 h, the CRCs of six TSMs are also S-shaped but the other 17 TSMs are non-monotonic hormetic or J-shaped at 12 h. In addition, to effectively characterize the nature of the stimulation and inhibition phases, it is suggested that five parameters such as the EC_L (the median stimulation effective concentration (left)), E_min (the maximum stimulation effect), EC_min (the maximum stimulation effective concentration), ZEP (zero effect point where the effect is 0 and the concentration is ZEP), and EC₅₀ can depict the non-monotonic CRC. To the best of our knowledge, this is the first study about the hormetic CRCs of commercial PCP mixtures.