Using standard additions to improve extraction and quantification of inositol hexakisphosphate in sediment samples by ion chromatography electrospray ionization mass spectrometry

Constantin Recknagel; Pernilla Thelin; Marion Abraham; Detlef Schulz-Bull; Per J R Sjöberg

doi:10.1016/j.talanta.2018.05.072

Using standard additions to improve extraction and quantification of inositol hexakisphosphate in sediment samples by ion chromatography electrospray ionization mass spectrometry

Talanta. 2018 Oct 1:188:192-198. doi: 10.1016/j.talanta.2018.05.072. Epub 2018 May 22.

Authors

Constantin Recknagel¹, Pernilla Thelin², Marion Abraham³, Detlef Schulz-Bull³, Per J R Sjöberg²

Affiliations

¹ Leibniz Institute for Baltic Sea Research Warnemuende, Department of Marine Chemistry, Seestrasse 15, 18119 Rostock, Germany. Electronic address: [email protected].
² Department of Chemistry - BMC, Uppsala University, P.O. Box 599, S-75124 Uppsala, Sweden.
³ Leibniz Institute for Baltic Sea Research Warnemuende, Department of Marine Chemistry, Seestrasse 15, 18119 Rostock, Germany.

PMID: 30029363
DOI: 10.1016/j.talanta.2018.05.072

Abstract

Several key aspects for the analysis of inositol hexakisphosphate (InsP₆) have been investigated in order to establish a suitable method for the study of sediment samples from different aquatic systems. Apparent matrix effects for the ion chromatography electrospray ionization tandem mass spectrometric detection (IC-ESI-MS/MS) method were accounted for with a standard addition approach, which also compensated for variation in extraction efficiency. Several parameters of the extraction method were optimized to improve the extraction efficiency for different sediment types. We observed an improvement in the extraction efficiency between 18% and 720%. Finally, the method was used to gain first insights into the relevance of InsP₆ in two aquatic systems located at the German Baltic coastal area. InsP₆ was detected in several sediment samples with concentrations between 2.3 and 15.2 µg InsP₆-P/g dry weight (DW).

Keywords: DWDry weight; IC-ESI-MS; Inositol phosphates; InsPnInositol phosphates; Sediment; Standard addition; TOCTotal organic carbon; TOPTotal organic phosphorus; TPTotal phosphorus.