Anti-Mycobacterium tuberculosis activity of naphthoimidazoles combined with isoniazid and rifampicin

Tuberculosis (Edinb). 2018 Jul:111:198-201. doi: 10.1016/j.tube.2018.06.015. Epub 2018 Jul 3.

Abstract

Tuberculosis (TB) is the cause of more than one million deaths worldwide, and despite being a curable disease, some factors can make therapy difficult, emphasizing the need for the development of new drugs that may potentiate the action of the classic anti-TB antimicrobials. Naphthoimidazoles show a broad spectrum of biological activities, including antimycobacterial activity. The aim of this study was to evaluate the anti-Mycobacterium tuberculosis activity of nine naphthoimidazoles, alone and combined with isoniazid (INH) and rifampicin (RIF). We evaluated the minimum inhibitory concentration (MIC) of the compounds, the fractional inhibitory concentration of the combinations of the naphthoimidazoles with INH or RIF, and the cytotoxicity of these compounds. Eight compounds showed MICs ranging from 1.56 to 25 μg/mL and the presence of substituents on phenyl groups shown to be essential for antimycobacterial activity. Four compounds showed additivity with both INH and RIF and showed SI values higher than 10, indicating safety. Thus, considering the antimycobacterial activity and the absence of antagonism between naphthoimidazoles and the two main drugs for TB treatment, these compounds could be scaffolds for the development of new anti-TB drugs.

Keywords: Naphthoimidazoles; New drugs; Tuberculosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antitubercular Agents / chemical synthesis
  • Antitubercular Agents / pharmacology*
  • Drug Discovery
  • Drug Resistance, Bacterial
  • Drug Therapy, Combination
  • Humans
  • Imidazoles / chemical synthesis
  • Imidazoles / pharmacology*
  • Isoniazid / pharmacology*
  • Microbial Sensitivity Tests
  • Molecular Structure
  • Mycobacterium tuberculosis / drug effects*
  • Mycobacterium tuberculosis / growth & development
  • Mycobacterium tuberculosis / pathogenicity
  • Naphthoquinones / chemical synthesis
  • Naphthoquinones / pharmacology*
  • Rifampin / pharmacology*
  • Structure-Activity Relationship
  • Tuberculosis / drug therapy*
  • Tuberculosis / microbiology

Substances

  • Antitubercular Agents
  • Imidazoles
  • Naphthoquinones
  • Isoniazid
  • Rifampin