Utility of pyrosequencing for rapid detection of tubercular meningitis (TBM) and associated susceptibility directly from CSF specimens

Kanchan Ajbani; Mubin Kazi; Swapna Naik; Rajeev Soman; Anjali Shetty; Camilla Rodrigues

doi:10.1016/j.tube.2018.05.009

Utility of pyrosequencing for rapid detection of tubercular meningitis (TBM) and associated susceptibility directly from CSF specimens

Tuberculosis (Edinb). 2018 Jul:111:54-56. doi: 10.1016/j.tube.2018.05.009. Epub 2018 May 19.

Authors

Kanchan Ajbani¹, Mubin Kazi¹, Swapna Naik¹, Rajeev Soman², Anjali Shetty¹, Camilla Rodrigues³

Affiliations

¹ P. D. Hinduja Hospital & Medical Research Centre, Dept. of Microbiology, Veer Sarvarkar Marg, Mahim, Mumbai, India.
² P. D. Hinduja Hospital & Medical Research Centre, Dept. of Medicine, Veer Sarvarkar Marg, Mahim, Mumbai, India.
³ P. D. Hinduja Hospital & Medical Research Centre, Dept. of Microbiology, Veer Sarvarkar Marg, Mahim, Mumbai, India. Electronic address: [email protected].

PMID: 30029915
DOI: 10.1016/j.tube.2018.05.009

Abstract

Tubercular meningitis (TBM) is a serious form of tuberculosis (TB). The diagnosis of TBM & susceptibility/resistance is difficult as TB MGIT culture lacks sensitivity & timeliness. Timely and accurate diagnosis of the TBM is the need of the hour for initiation of appropriate therapy. We have exploited pyrosequencing to detect TB and associated Multi/extensively drug resistant (MDR/ XDR-TB) directly from Cerebrospinal Fluid (CSF) specimens.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Adolescent
Adult
Antitubercular Agents / therapeutic use
Bacteriological Techniques*
Cerebrospinal Fluid / microbiology
DNA, Bacterial / cerebrospinal fluid
DNA, Bacterial / genetics*
Drug Resistance, Multiple, Bacterial / genetics*
Extensively Drug-Resistant Tuberculosis / cerebrospinal fluid
Extensively Drug-Resistant Tuberculosis / diagnosis*
Extensively Drug-Resistant Tuberculosis / drug therapy
Extensively Drug-Resistant Tuberculosis / microbiology
Female
Genotype
High-Throughput Nucleotide Sequencing*
Humans
Male
Middle Aged
Mycobacterium tuberculosis / drug effects
Mycobacterium tuberculosis / genetics*
Mycobacterium tuberculosis / pathogenicity
Phenotype
Predictive Value of Tests
Reproducibility of Results
Sequence Analysis, DNA / methods*
Time Factors
Tuberculosis, Meningeal / cerebrospinal fluid
Tuberculosis, Meningeal / diagnosis*
Tuberculosis, Meningeal / drug therapy
Tuberculosis, Meningeal / microbiology
Tuberculosis, Multidrug-Resistant / cerebrospinal fluid
Tuberculosis, Multidrug-Resistant / diagnosis*
Tuberculosis, Multidrug-Resistant / drug therapy
Tuberculosis, Multidrug-Resistant / microbiology
Workflow
Young Adult

Substances

Antitubercular Agents
DNA, Bacterial