Abstract
Rho GTPases are Ras-family G proteins that regulate many critical cellular functions. Due to their requirement during early embryonic development, investigations into the function of Rho GTPases at a tissue-specific level require inducible and spatially targeted modulation of Rho GTPase activity. Here, we describe the use of ten novel zebrafish transgenics enabling GAL4-specific expression of Rho GTPases to modulate Rho GTPase activity with spatial and temporal control.
Keywords:
Cdc42; GAL4/UAS; Heat shock; Rac1; RhoA; Transgenics; Zebrafish.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Animals, Genetically Modified* / embryology
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Animals, Genetically Modified* / genetics
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DNA-Binding Proteins* / genetics
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DNA-Binding Proteins* / metabolism
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Embryo, Nonmammalian / embryology*
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Embryonic Development*
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Gene Expression Regulation, Developmental
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Gene Expression Regulation, Enzymologic
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Transcription Factors* / genetics
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Transcription Factors* / metabolism
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Zebrafish Proteins* / genetics
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Zebrafish Proteins* / metabolism
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Zebrafish* / embryology
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Zebrafish* / genetics
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rho GTP-Binding Proteins* / biosynthesis
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rho GTP-Binding Proteins* / genetics
Substances
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DNA-Binding Proteins
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Gal4 protein, zebrafish
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Transcription Factors
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Zebrafish Proteins
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rho GTP-Binding Proteins