Influenza A(H5N1) viruses with A(H9N2) single gene (matrix or PB1) reassortment isolated from Cambodian live bird markets

Annika Suttie; Erik A Karlsson; Yi-Mo Deng; Srey Viseth Horm; Sokhoun Yann; Songha Tok; San Sorn; Davun Holl; Sothyra Tum; Aeron C Hurt; Andrew R Greenhill; Ian G Barr; Paul F Horwood; Philippe Dussart

doi:10.1016/j.virol.2018.07.028

Influenza A(H5N1) viruses with A(H9N2) single gene (matrix or PB1) reassortment isolated from Cambodian live bird markets

Virology. 2018 Oct:523:22-26. doi: 10.1016/j.virol.2018.07.028. Epub 2018 Jul 31.

Authors

Affiliations

¹ Virology Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, Phnom Penh, Cambodia; School of Applied and Biomedical Sciences, Federation University, Churchill, Australia; WHO Collaborating Centre for Reference and Research on Influenza, Victorian Infectious Diseases Reference Laboratory, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria 3000, Australia.
² Virology Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, Phnom Penh, Cambodia.
³ WHO Collaborating Centre for Reference and Research on Influenza, Victorian Infectious Diseases Reference Laboratory, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria 3000, Australia.
⁴ General Directorate for Animal Health and Production, Cambodian Ministry of Agriculture, Forestry and Fisheries, Phnom Penh, Cambodia.
⁵ National Animal Health and Production Research Institute, Cambodian Ministry of Agriculture, Forestry and Fisheries, Phnom Penh, Cambodia.
⁶ School of Applied and Biomedical Sciences, Federation University, Churchill, Australia.
⁷ Virology Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, Phnom Penh, Cambodia; Australian Institute of Tropical Health and Medicine, James Cook University, Cairns, Queensland, 4870, Australia. Electronic address: [email protected].
⁸ Virology Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, Phnom Penh, Cambodia. Electronic address: [email protected].

PMID: 30075357
DOI: 10.1016/j.virol.2018.07.028

Abstract

Live bird market surveillance for avian influenza viruses in Cambodia in 2015 has led to the detection of two 7:1 reassortant influenza A(H5N1) clade 2.3.2.1c viruses. These reassortant strains, designated A/duck/Cambodia/Z564W35M1/2015 and A/chicken/Cambodia/Z850W49M1/2015, both contained a single gene (PB1 and matrix gene, respectively) from concurrently circulating A(H9N2) influenza viruses. All other viral genes from both isolates clustered with A(H5N1) clade 2.3.2.1 viruses. Continued and prolonged co-circulation of influenza A(H5N1) and A(H9N2) viruses in Cambodian live bird markets may present a risk for the emergence of novel influenza reassortant viruses with negative agricultural and/or public health implications.

Keywords: A(H5N1); A(H9N2); Cambodia; Influenza; Live bird markets; Reassortment.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Cambodia / epidemiology
Chickens
Ducks
Epidemiological Monitoring
Gene Expression
Influenza A Virus, H5N1 Subtype / classification
Influenza A Virus, H5N1 Subtype / genetics*
Influenza A Virus, H5N1 Subtype / isolation & purification
Influenza A Virus, H5N1 Subtype / metabolism
Influenza A Virus, H9N2 Subtype / classification
Influenza A Virus, H9N2 Subtype / genetics*
Influenza A Virus, H9N2 Subtype / isolation & purification
Influenza A Virus, H9N2 Subtype / metabolism
Influenza in Birds / epidemiology*
Influenza in Birds / transmission
Influenza in Birds / virology
Phylogeny
Poultry Diseases / epidemiology*
Poultry Diseases / transmission
Poultry Diseases / virology
Reassortant Viruses / classification
Reassortant Viruses / genetics*
Reassortant Viruses / isolation & purification
Reassortant Viruses / metabolism
Viral Matrix Proteins / genetics
Viral Matrix Proteins / metabolism
Viral Proteins / genetics
Viral Proteins / metabolism

Substances

M1 protein, Influenza A virus
Viral Matrix Proteins
Viral Proteins
influenza virus polymerase basic protein 1