Resequencing of four novel alleles with nanopore technology

HLA. 2018 Oct;92(4):233-234. doi: 10.1111/tan.13360. Epub 2018 Sep 4.

Authors

D Fuerst^{1

2}, F Radecke², H Schrezenmeier^{1

2}, K Schwarz^{1

2}, J Mytilineos^{1

2}

Affiliations

¹ Institute for Clinical Transfusion Medicine and Immunogenetics Ulm, German Red Cross Blood Transfusion Service, Baden Wuerttemberg-Hessen and University Hospital Ulm, Ulm, Germany.
² Institute of Transfusion Medicine, University of Ulm, Ulm, Germany.

PMID: 30079498
DOI: 10.1111/tan.13360

Abstract

Long nanopore reads allow phasing of heterozygous positions but show limitations in sequencing of homopolymers.

Keywords: HLA; ambiguities; nanopore; phasing.

© 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

MeSH terms

Alleles
Genotyping Techniques / methods*
High-Throughput Nucleotide Sequencing / methods*
Histocompatibility Antigens Class I / genetics*
Histocompatibility Antigens Class II / genetics*
Humans
Nanopores*
Sequence Analysis, DNA / methods*

Substances

Histocompatibility Antigens Class I
Histocompatibility Antigens Class II