A Green BODIPY-Based, Super-Fluorogenic, Protein-Specific Labelling Agent

Angew Chem Int Ed Engl. 2018 Sep 17;57(38):12390-12394. doi: 10.1002/anie.201805482. Epub 2018 Aug 24.

Abstract

We report the development of YC23, a novel green BODIPY-based dimaleimide derivative that undergoes a fluorogenic addition reaction (FlARe) with a genetically encodable peptide tag (dC10α) that can be fused to a protein of interest (POI). We also demonstrate the application of this reaction for the fluorogenic labelling of a specific POI in bacterial lysate and in living mammalian cells.

Keywords: BODIPY; fluorogenic probes; maleimide; protein labelling.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Boron Compounds / chemistry*
  • Fluorescent Dyes / chemistry*
  • HeLa Cells
  • Histones / genetics
  • Histones / metabolism
  • Humans
  • Maleimides / chemistry
  • Maltose-Binding Proteins / genetics
  • Maltose-Binding Proteins / metabolism
  • Microscopy, Fluorescence
  • Peptides / chemistry
  • Peptides / genetics
  • Peptides / metabolism*

Substances

  • 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
  • Boron Compounds
  • Fluorescent Dyes
  • Histones
  • Maleimides
  • Maltose-Binding Proteins
  • Peptides

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