In-capillary microextraction for direct mass spectrometry analysis of biological samples

Talanta. 2018 Nov 1:189:451-457. doi: 10.1016/j.talanta.2018.07.027. Epub 2018 Jul 11.

Abstract

Slug flow microextraction (SFME)-nanoESI was originally explored as a single-step sampling ionization method for MS analysis of biofluids. In this work, a comprehensive study and development of the SFME has been carried out. Revers-phase SFME was developed to analyze chemical compounds in oil samples. A three-phase SFME system was introduced as a suitable approach for analyzing polar compounds in biofluids. The impacts by the capillary inner diameter, solvent and sample properties were also investigated, leading the use of fused silica capillaries for performing SFME.

Keywords: Biofluid; Mass spectrometry; Polar compounds; Slug flow microreaction.

MeSH terms

  • Body Fluids / chemistry*
  • Hydrophobic and Hydrophilic Interactions
  • Liquid Phase Microextraction / methods*
  • Nanotechnology
  • Oils / chemistry
  • Solvents / chemistry
  • Spectrometry, Mass, Electrospray Ionization / methods*

Substances

  • Oils
  • Solvents