Abstract
The levels of fructose 2,6-P2 and 6-phosphofructo 2-kinase have been found to be decreased in the liver of both ketotic and non-ketotic diabetic rats, a good correlation between fall of hepatic fructose 2,6-P2, ketonemia and glycemia being observed. The "total" 6-phosphofructo 2-kinase activity and the "active" (non-phosphorylated) from of the enzyme were decreased to a different extent, resulting in a fall of the "active"/"total" activity ratio. Hepatic levels of glucose 1,6-P2 were lowered only in ketotic diabetes. Insulin treatment normalized all the values studied. Insulin administration to control rats decreased the hepatic levels of fructose 2,6-P2 and did not affect glucose 1,6-P2 levels. It also decreased the "active" form of 6-phosphofructo 2-kinase, without significantly altering the "total" activity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3-Hydroxybutyric Acid
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Animals
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Blood Glucose / metabolism
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Diabetes Mellitus, Experimental / drug therapy
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Diabetes Mellitus, Experimental / metabolism*
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Diabetic Ketoacidosis / metabolism
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Enzyme Activation
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Fructosediphosphates / metabolism*
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Glucose-6-Phosphate* / analogs & derivatives*
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Glucosephosphates / metabolism*
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Hexosediphosphates / metabolism*
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Hydroxybutyrates / blood
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Insulin / therapeutic use
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Liver / metabolism*
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Male
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Phosphofructokinase-2
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Phosphorylation
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Phosphotransferases / metabolism*
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Rats
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Rats, Inbred Strains
Substances
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Blood Glucose
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Fructosediphosphates
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Glucosephosphates
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Hexosediphosphates
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Hydroxybutyrates
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Insulin
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Glucose-6-Phosphate
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fructose 2,6-diphosphate
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glucose-1,6-bisphosphate
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Phosphotransferases
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Phosphofructokinase-2
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3-Hydroxybutyric Acid