Enhancement of the adaptability of anammox granules to zinc shock by appropriate organic carbon treatment

Heavy metals, which are commonly present in high ammonia-containing wastewater, can cause inhibitory effects to anammox reaction. This study proposes a novel approach to enhance the adaptability of anammox granules to heavy metal [Zn(II)] shock by organic carbon (sodium acetate, NaAc) treatment, paying special attention to optimization of the treatment dosage and duration. For granules treated with 200 mg chemical oxygen demand (COD)/L NaAc for 2 d, the activity recovery (six cycles) efficiency after Zn(II) (40 mg/L) shock reached 127.4%. The extracellular polymeric substance (EPS) production increased by 168% and heterotrophic bacteria mildly proliferated (increased by 14%) in such granules compared with the control. The dramatic recovery capacity was likely due to the entrapment and barrier function of EPS and the outer-layer proliferated heterotrophic bacteria. This finding offers a useful process to enable maximum adaptability of anammox granules from heavy metals shocks, allowing anammox technology to be more widely applied.