Acquisition of antimicrobial-resistant variants in repeated infections caused by Pseudomonas aeruginosa revealed by whole genome sequencing

Noriko Nakanishi; Ryohei Nomoto; Kanako Sato; Chihiro Koike; Mari Kusuki; Tatsuya Nakamura; Katsumi Shigemura; Toshiro Shirakawa; Masato Fujisawa; Issei Tokimatsu; Kayo Osawa

doi:10.1016/j.jiac.2018.07.016

Acquisition of antimicrobial-resistant variants in repeated infections caused by Pseudomonas aeruginosa revealed by whole genome sequencing

J Infect Chemother. 2019 Feb;25(2):154-156. doi: 10.1016/j.jiac.2018.07.016. Epub 2018 Aug 17.

Authors

Affiliations

¹ Department of Infectious Diseases, Kobe Institute of Health, Kobe, Japan.
² Department of Biophysics, Kobe University Graduate School of Health Sciences, Kobe, Japan.
³ Department of Infection Prevention and Control, Kobe University Hospital, Kobe, Japan; Department of Clinical Laboratory, Kobe University Hospital, Kobe, Japan.
⁴ Department of Infection Prevention and Control, Kobe University Hospital, Kobe, Japan; Division of Urology, Department of Organ Therapeutics, Faculty of Medicine, Kobe University Graduate School of Medicine, Kobe Japan.
⁵ Division of Urology, Department of Organ Therapeutics, Faculty of Medicine, Kobe University Graduate School of Medicine, Kobe Japan; Division of Translational Research for Biologics, Department of Internal Medicine Related, Kobe University Graduate School of Medicine, Kobe, Japan.
⁶ Division of Urology, Department of Organ Therapeutics, Faculty of Medicine, Kobe University Graduate School of Medicine, Kobe Japan.
⁷ Department of Infection Prevention and Control, Kobe University Hospital, Kobe, Japan.
⁸ Department of Biophysics, Kobe University Graduate School of Health Sciences, Kobe, Japan; Department of Infection Prevention and Control, Kobe University Hospital, Kobe, Japan. Electronic address: [email protected].

PMID: 30126693
DOI: 10.1016/j.jiac.2018.07.016

Abstract

Pseudomonas aeruginosa, responsible for serious nosocomial-acquired infections, possesses intrinsic antibiotic resistance mechanisms and commonly exhibits multidrug resistance. Here, we report the evolving resistance profiles of strains isolated from the sputum of a patient being treated for repeated P. aeruginosa infections following cancer resection. Whole genome sequencing of six isolates obtained over a 2-month period revealed two key single nucleotide polymorphisms in the mexR and gyrB genes that affected efflux pump expression and antimicrobial resistance.

Keywords: Antibiotic resistance; Pseudomonas aeruginosa; SNP; WGS; mexR.

MeSH terms

Anti-Bacterial Agents / pharmacology
Bacterial Proteins / genetics
Colonic Neoplasms / surgery
Drug Resistance, Bacterial / genetics*
Humans
Microbial Sensitivity Tests
Polymorphism, Single Nucleotide / genetics
Pseudomonas Infections / microbiology*
Pseudomonas aeruginosa / drug effects*
Pseudomonas aeruginosa / genetics*
Repressor Proteins / genetics
Whole Genome Sequencing

Substances

Anti-Bacterial Agents
Bacterial Proteins
MexR protein, Pseudomonas aeruginosa
Repressor Proteins