Absence of Persistent Hepatitis E Virus Infection in Antibody-Deficient Patients Is Associated With Transfer of Antigen-Neutralizing Antibodies From Immunoglobulin Products

J Infect Dis. 2019 Jan 7;219(2):245-253. doi: 10.1093/infdis/jiy504.

Abstract

Background: Persistent hepatitis E virus (HEV) infection is described in a number of immunosuppressive conditions. We aimed to determine the risk of persistent HEV infection in patients with primary or secondary antibody deficiency.

Methods: Two hundred forty-five antibody-deficient patients receiving regular immunoglobulin replacement therapy were tested for HEV RNA and anti-HEV immunoglobulin G (IgG). Immunoglobulin products and plasma specimens obtained from 9 antibody-deficient patients before and after intravenous immunoglobulin (IVIG) therapy, 5 recently treated patients with persistent HEV infection, and 5 healthy patients recovered from acute HEV infection were analyzed for anti-HEV IgG and for antibody reacting with HEV antigen.

Results: No antibody-deficient patient had detectable plasma HEV RNA. Anti-HEV IgG was detected in 38.8% of patients. All 10 immunoglobulin products tested contained anti-HEV capable of neutralizing HEV antigen. Plasma samples collected following IVIG infusion therapy demonstrated a higher anti-HEV IgG level and neutralizing activity, compared with samples collected before IVIG therapy. Neutralizing activity was similar to that in healthy patients with recent acute HEV infection.

Conclusion: The risk of persistent HEV infection in patients with antibody deficiency appears extremely low. This may be due to passive seroprotection afforded by the ubiquitous presence of anti-HEV in immunoglobulin replacement products.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Antibodies, Neutralizing / immunology*
  • Cross-Sectional Studies
  • Female
  • Hepatitis Antibodies / blood
  • Hepatitis Antibodies / therapeutic use*
  • Hepatitis E / immunology*
  • Hepatitis E / therapy*
  • Hepatitis E virus
  • Humans
  • Immunoglobulin G / blood
  • Immunoglobulin M / blood
  • Immunoglobulins, Intravenous / blood
  • Immunoglobulins, Intravenous / therapeutic use*
  • Immunologic Deficiency Syndromes / complications
  • Immunologic Deficiency Syndromes / immunology*
  • Male
  • Middle Aged
  • RNA, Viral / blood

Substances

  • Antibodies, Neutralizing
  • Hepatitis Antibodies
  • Immunoglobulin G
  • Immunoglobulin M
  • Immunoglobulins, Intravenous
  • RNA, Viral